KMID : 0613820020120030274
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Journal of Life Science 2002 Volume.12 No. 3 p.274 ~ p.280
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Phenylalanine Ammonia Lyase and Cinnamic Acid 4-Hydroxylase Activities of Rice and Pepper in response to UV and Wounding
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Kim Mi-Young
Yoon Yong-Hwi Lee Jung-Hoon Kim Hak-Yoon Shin Dong-Hyun Lee Jin-Hyung Kim Dal-Ung Kim Kil-Ung
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Abstract
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The metabolites related to phenylpropanoid pathway play an important role in the self-defense of plants and induced by environmental stress like wounding, pathogen attack, UV-irradiation and so on. The mRNA level of rice phenylalanine ammonia lyase (PAL) was increased at 12 h to 48 h, however it was gradually decreased 48 h to 60 h after UV irradiation. The PAL enzyme activities in rice were peaked at the time of 24 h after UV irradiation, on the other hand, it was not affected by wounding. The PAL enzyme activities in pepper were raised high at 24 h and 10 h by UV irradiation and wounding respectively. The cinnamic acid 4-hydroxylase (C4H) activities were increased by wounding treatment and were detected from 12 h to end time point of experiment, while UV-irradiation didn¡¯t affect the C4H activity in rice and pepper. These results were assumed that the action of isoflavonid has an alternative effect on the defenses which include wounding and UV irradiation and on the diverse roles in rice and hot pepper.
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KEYWORD
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cinnamic acid 4-hydroxylase (C4H), pepper, phenylalanine ammonia lyase (PAL), rice
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